Answer of Dermatopathology Case 72
Cutaneous Leishmaniasis Visit: Dermatopathology site Visit: Pathology of Leishmaniasis Abstract: Molecular characterization of leishmania species isolated from cutaneous leishmaniasis in yemen. PLoS One.2010 Sep 20;5(9). pii: e12879. BACKGROUND: Cutaneous leishmaniasis (CL) is a neglected tropical disease endemic in the tropics and subtropics with a global yearly incidence of 1.5 million. Although CL is the most common form of leishmaniasis, which is responsible for 60% of DALYs lost due to tropical-cluster diseases prevalent in Yemen, available information is very limited. METHODOLOGY/PRINCIPAL FINDINGS: This study was conducted to determine the molecular characterization of Leishmania species isolated from human cutaneous lesions in Yemen. Dermal scrapes were collected and examined for Leishmania amastigotes using the Giemsa staining technique. Amplification of the ribosomal internal transcribed spacer 1(ITS-1) gene was carried out using nested PCR and subsequent sequencing. The seq